可靠方法 采用抗人膀胱癌单克隆抗体BDI-1通过2-巯基乙醇直接还原法标记99mTc(99mTc)制备了99mTc-BDI-

　　1。结果 标记率为69.9％，放化纯度大于95％。33例患者接受了通过膀胱内灌注的放射免疫显像诊断，其中4例非膀胱癌患者均未见显像，29例膀胱癌患者肿瘤显像阳性率为88.5％。

　　原发和复发肿瘤均可获得清晰的显像，显像肿瘤最小为0.5cm，肿瘤细胞分级包括G1~G3。

　　显像结果与膀胱镜检结果完全符合者占65.2％，11例多发肿瘤中有7例（63.6％）显像结果在肿瘤数目、位置及大小上与膀胱镜检有所差异。结论 该方法为膀胱癌的诊断提供了一种的安全有效的手段。

　　【关键词】 膀胱肿瘤 抗体，单克隆 放射免疫检测Radioimmunoimaging of bladder tumor with the intravesical administration of techcomium -99m labelled

　　monoclonal antibody Li Ningchen，Yu Lizhang，Zhang Chunli，et al.Institute of Urology，Department of Urolo

　　gy，First Hospital of Beijing Medical University，Beijing 100034.

　　【Abstract】 Objective To make radioimmunoimaging of bladder tumor simple，safe and reliable.

　　methods Anti-human bladder carcinoma cell line BIU-87 monoclonal antibody-BDI-1 was labelled with

　　techcomium -99m by direct mercaptoethanol reducing method.Results the labelling rate was 69.6% and the radiochemistry pruity was higher than 95%. 33 patients received radioimmunoimaging of bladder tumor with

　　the intravesical administration of techcomium-99m labelled monoclonal antibody.The false positive rate was

　　zero.88.5% of all 29 bladder cancer patients had got positive immunoscintigraphy results， and 65.2% of

　　them were confirmed by cystoscopic results.Both primary and recurrent tumor could be detected clearly

　　and the smallest tumor detected was 0.5cm in diameter.There were some differences between the

　　radioimmunoimaging and cystoscopic results in 7 of 11 multitumor cases，which included tumor size，number

　　and location.Conclusion It is required to use more suitable isotopes and/or monoclonal antibodies to

　　increase its sensitivity.